Establishment of Lipofection Protocol for Efficient miR-21 Transfection into Cortical Neurons In Vitro
نویسندگان
چکیده
منابع مشابه
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
A DNA-transfection protocol has been developed that makes use of a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). Small unilamellar liposomes containing DOTMA interact spontaneously with DNA to form lipid-DNA complexes with 100% entrapment of the DNA, DOTMA facilitates fusion of the complex with the plasma membrane of tissue culture cells, resul...
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ژورنال
عنوان ژورنال: DNA and Cell Biology
سال: 2015
ISSN: 1044-5498,1557-7430
DOI: 10.1089/dna.2015.2800